{ "id": "volcano-plot-differential-expression", "schemaVersion": 2, "title": { "ja": "処置後の遺伝子発現 — 上昇4、下降3が基準超え", "en": "Differential Expression — Four Up and Three Down Pass the Thresholds" }, "summary": { "ja": "効果量とp値を二軸にし、閾値の外にある有意な上昇・下降候補を分類するvolcano plot", "en": "A volcano plot classifies significant increases and decreases using effect-size and p-value thresholds on two axes." }, "capturedAt": "2026-09-25", "categories": [ "presentation" ], "tags": [ "data-viz", "minimal" ], "useCases": [ "comparison", "comparison" ], "format": "slide-16x9", "density": "medium", "layout": { "kind": "axis", "canvas": { "w": 1920, "h": 1080 }, "margin": { "top": 100, "right": 140, "bottom": 100, "left": 140 } }, "type": { "title": { "size": 46, "weight": 700, "tracking": "0.01em" }, "subtitle": { "size": 22, "weight": 500 }, "axisTitle": { "size": 22, "weight": 700 }, "tick": { "size": 20, "weight": 500 }, "axisLabel": { "size": 20, "weight": 500 }, "gene": { "size": 20, "weight": 500 }, "legend": { "size": 20, "weight": 500 }, "takeaway": { "size": 28, "weight": 500 }, "note": { "size": 20, "weight": 400 } }, "colors": { "ground": "#FFFFFF", "surface": "#F4F6F9", "ink": "#131820", "muted": "#667183", "accent": "#1F5FA8", "rule": "#DBE1E9", "series": [] }, "rules": [ { "ja": "横軸はlog₂ fold change、縦軸は−log₁₀(p値)。上ほど統計的有意性が高い", "en": "Plot log₂ fold change on x and −log₁₀(p) on y; points higher up have stronger statistical significance." }, { "ja": "縦横の閾値線で区切り、有意かつ効果量基準を超える上昇・下降を分ける", "en": "Threshold lines separate significant changes that also pass the effect-size cutoff into increases and decreases." }, { "ja": "一般的な散布図の相関解釈ではなく、仮説検定と変化方向の候補抽出に使う", "en": "Use this to screen tested changes by direction, not to interpret a generic correlation between two variables." }, { "ja": "架空の12遺伝子。実分析では多重検定補正と実験条件を併せて判断する", "en": "Twelve fictional genes; real analyses also require multiple-testing correction and experimental context." } ], "cover": "https://design-vault.pages.dev/_astro/cover.mjFyUypN.png", "assets": [], "shortlist": false, "status": "published", "relations": { "similar": [] }, "license": "Original", "publishedAt": null, "reproduction": { "spec": { "format": "slide-16x9", "density": "medium", "layout": { "kind": "axis", "canvas": { "w": 1920, "h": 1080 }, "margin": { "top": 100, "right": 140, "bottom": 100, "left": 140 } }, "type": { "title": { "size": 46, "weight": 700, "tracking": "0.01em" }, "subtitle": { "size": 22, "weight": 500 }, "axisTitle": { "size": 22, "weight": 700 }, "tick": { "size": 20, "weight": 500 }, "axisLabel": { "size": 20, "weight": 500 }, "gene": { "size": 20, "weight": 500 }, "legend": { "size": 20, "weight": 500 }, "takeaway": { "size": 28, "weight": 500 }, "note": { "size": 20, "weight": 400 } }, "colors": { "ground": "#FFFFFF", "surface": "#F4F6F9", "ink": "#131820", "muted": "#667183", "accent": "#1F5FA8", "rule": "#DBE1E9" }, "rules": [ "Plot log₂ fold change on x and −log₁₀(p) on y; points higher up have stronger statistical significance.", "Threshold lines separate significant changes that also pass the effect-size cutoff into increases and decreases.", "Use this to screen tested changes by direction, not to interpret a generic correlation between two variables.", "Twelve fictional genes; real analyses also require multiple-testing correction and experimental context." ] }, "prompt": { "ja": "以下の仕様でスライドを 1 枚作ってください。\n\n- 版面 1920 × 1080\n- 余白 上 100 右 140 下 100 左 140\n\n## 文字（版面 1920px に対する px）\ntitle 46px / 700 / 字間 0.01em\nsubtitle 22px / 500\naxisTitle 22px / 700\ntick 20px / 500\naxisLabel 20px / 500\ngene 20px / 500\nlegend 20px / 500\ntakeaway 28px / 500\nnote 20px / 400\n\n## 配色\n地 #FFFFFF / 面 #F4F6F9 / 文字 #131820 / 補助 #667183 / 強調 #1F5FA8 / 罫 #DBE1E9\n\n## 外すと崩れる点\n- 横軸はlog₂ fold change、縦軸は−log₁₀(p値)。上ほど統計的有意性が高い\n- 縦横の閾値線で区切り、有意かつ効果量基準を超える上昇・下降を分ける\n- 一般的な散布図の相関解釈ではなく、仮説検定と変化方向の候補抽出に使う\n- 架空の12遺伝子。実分析では多重検定補正と実験条件を併せて判断する\n\n内容は［ここに自分の内容を書く］に置き換えてください。寸法と配色はそのまま守ってください。\n正確に再現する場合は、この HTML をそのまま使ってください: https://design-vault.pages.dev/designs/volcano-plot-differential-expression/pattern.html", "en": "Produce one slide to the following specification.\n\n- Canvas 1920 × 1080\n- Margin top 100 right 140 bottom 100 left 140\n\n## Type (px against a 1920px canvas)\ntitle 46px / 700 / tracking 0.01em\nsubtitle 22px / 500\naxisTitle 22px / 700\ntick 20px / 500\naxisLabel 20px / 500\ngene 20px / 500\nlegend 20px / 500\ntakeaway 28px / 500\nnote 20px / 400\n\n## Colour\nGround #FFFFFF / Surface #F4F6F9 / Ink #131820 / Muted #667183 / Accent #1F5FA8 / Rule #DBE1E9\n\n## Constraints — dropping these breaks it\n- Plot log₂ fold change on x and −log₁₀(p) on y; points higher up have stronger statistical significance.\n- Threshold lines separate significant changes that also pass the effect-size cutoff into increases and decreases.\n- Use this to screen tested changes by direction, not to interpret a generic correlation between two variables.\n- Twelve fictional genes; real analyses also require multiple-testing correction and experimental context.\n\nReplace the content with [your content]. Keep the measurements and colours as given.\nFor an exact reproduction, use the verbatim HTML at https://design-vault.pages.dev/designs/volcano-plot-differential-expression/pattern.html." }, "code": { "html": "\n\n処置後の遺伝子発現 — 上昇4、下降3が基準超え\n\n\n\n
\n\n\n\n処置後の遺伝子発現 — 上昇4、下降3が基準超え\n架空の12遺伝子 / 閾値: |log₂ fold change| ≥ 1、p値 < 0.05\n\n効果量と統計的な有意性を合わせ、候補の方向を分類\n−log₁₀(p)\n\n\n\n\n\np=.05\n012345\n−4−2−10+124\n\n\n\n\nAlphaBetaThetaMuDeltaEpsilonLambda\nlog₂ fold change（負:発現低下 / 正:発現上昇）\n有意な上昇有意な下降両閾値未達\n12候補中7遺伝子が両基準を超過 — 上昇4、下降3\n閾値は説明用。多重検定補正、実験計画、効果量の実用性を確認し、生物学的結論と同一視しない。\n
\n", "url": "https://design-vault.pages.dev/designs/volcano-plot-differential-expression/pattern.html", "fonts": [ "https://design-vault.pages.dev/designs/volcano-plot-differential-expression/font.css", "https://design-vault.pages.dev/designs/volcano-plot-differential-expression/font-0.woff2", "https://design-vault.pages.dev/designs/volcano-plot-differential-expression/font-1.woff2", "https://design-vault.pages.dev/designs/volcano-plot-differential-expression/font-2.woff2" ] } }, "urls": { "ja": "https://design-vault.pages.dev/designs/volcano-plot-differential-expression", "en": "https://design-vault.pages.dev/en/designs/volcano-plot-differential-expression" }, "notesMarkdown": "### 視覚的な問い\n\n処置後に発現が大きく変化し、統計的な基準も満たす遺伝子候補はどれか。上昇・下降の方向は何か。\n\n### 構造\n\n横軸はlog₂ fold change、縦軸は−log₁₀(p値)。縦の2本線が効果量の閾値±1、横線がp値0.05に相当する1.3を\n示す。両条件を満たす点を上昇（右・青）と下降（左・橙）に分け、基準未達は灰色にする。この架空例では12候補中\n7候補が基準を超え、内訳は上昇4・下降3。\n\n一般的な散布図は2変数の関係を見るが、volcano plotは実験条件間の変化量と検定結果で候補を選ぶ。p値は例示上\n未補正であり、実際の遺伝子発現分析では多重検定補正、反復数、実験設計と効果量の実用性を合わせて評価する。" }